Salt Solutions
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Artificial Seawater
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| Ingredients | ||
| NaCl |
28.32 gm
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| KCl |
0.77 gm
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| MgCl2·6H2O |
5.41 gm
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| MgSO4·7H2O |
7.13 gm
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| CaCl2 |
1.18 gm
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Directions |
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Dissolve in l liter distilled water. Add 0.2 gm NaHCO3 Adjust to pH 8.2, if necessary.
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Chick Embryo Ringer’s Solution
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| Ingredients | ||
| NaCl |
7.20 gm
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| KCl |
0.37 gm
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| CaCl2 |
0.17 gm
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Directions |
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Dissolve in 1 liter distilled water . |
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Holtfreter’s Solution (for Amphibian Embryos)
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| Ingredients | ||
| NaCl |
0.35 gm
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| KCl |
0.005 gm
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| CaCl2 |
0.01 gm
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| NaHCO3 |
0.02 gm
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Directions |
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| Dissolve in 100 ml distilled water.
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Insect Ringer’s Solution
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| Ingredients | ||
| NaCl |
7.5 gm
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| KCl |
0.35 gm
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| CaCl2 |
0.21 gm
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Directions |
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Dissolve in 1 liter distilled water . |
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Sodium-Free Seawater
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| Ingredients | ||
| Glycerol |
84.60 ml
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| MgCl2·6H2O |
11.20 gm
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| KCl |
0.77 gm
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| NaHCO3 |
0.21 gm
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| CaCl2 |
0.12 gm
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Directions |
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| Dissolve in l liter distilled water. | ||