Stains for Wholemounts
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Aceto-Orcein Stain
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| Ingredients | ||
| orcein |
1 gm
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| glacial acetic acid |
50.0 ml
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| 85% lactic acid |
50.0 ml
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Directions |
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Warm gently (do not boil), Used to stain chromosomes in chromosome squashes of Drosophila salivary glands. Stain for 2-5 min. prior to making the squash. |
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Mayer’s Carmalum
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| Ingredients | ||
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Stock solution:
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| carmine |
1 gm
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ammonium alum (aluminum ammonium |
10 gm
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| distilled water |
200 ml
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| Boil 20 minutes. | ||
| Working solution: | ||
| carmalum stock solution |
5 ml
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| glacial acetic acid |
0.4 ml
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| distilled water |
100 ml
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Directions |
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Mix ingredients for working solution. When dissolved, filter. Add 1 ml formalin to inhibit mold growth. Will keep indefinitely. Stains nuclei. Is a progressive stain. Specimens are usually adequately stained in 4-8 hours. Stop staining when specimen is a deep pink-red color. |
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